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KMID : 0379520070230010025
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한국독성학회지
2007 Volume.23 No. 1 p.25 ~ p.31
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Evaluation of Protective Effects of Houttuynia cordata on H_2O_2-Induced Oxidative DNA Damage Using an Alkaline Comet Assay in Human HepG2 Cells
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Hah Dae-Sik
Kim Chung-Hui
Lue Jae-Du
Kim Eui-Kyung
Kim Jong-Shu
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Abstract
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To evaluate the protective effect of Houttuynia cordata on hydrogen peroxide-induced oxidative DNA damage in HepG2 cell line, we used an alkaline single-cell gel electrophoresis (SCGE; comet assay). The DNA damage was analyzed by tail moment (TM) and tail length (TL), which used markers of DNA strand breaks in SCGE. The 100{mu}g/ml of methanolic extract of Houttuynia cordata root showed significant protective effects (p < 0.01) against hydrogen peroxide-induced DNA damage in HepG2 cells and increased cell viability against hydrogen peroxide. The results of this study indicate that Houttuynia cordata root methanol extract acts as a potential antioxidant, and exhibits potential anticancer properties, which may provide a clue to find applications in new pharmaceuticals for oxidative stability.
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KEYWORD
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DNA damage, Single cell gel electrophoresis (SCGE), Comet assay, Houttuynia cordota
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